propidium iodide staining  (Elabscience Biotechnology)

Journal: Microbiology Spectrum

Article Title: Helicobacter pylori inhibition by antimicrobial photodynamic therapy of methylene blue and porphyrin

doi: 10.1128/spectrum.03827-25

Figure Lengend Snippet: Inhibitory effects of MB and porphyrin on H. pylori free-swimming cells in vitro . ( A ) CFU of H. pylori treated with MB under 660 nm irradiation, ( B ) CFU of H. pylori when endogenous porphyrin was activated by 410 nm light, ( C ) live and dead cell counts of H. pylori treated with MB under 660 nm irradiation, and ( D ) live and dead cell counts of H. pylori when endogenous porphyrin was activated by 410 nm light. The SYTO 9 and propidium iodide were used to stain live and dead cells of H. pylori in flow cytometry. Experiments were repeated three times, statistics were analyzed using one-way analysis of variance ( A and C ) and Student’s t -test ( B and D ), * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: When H. pylori biofilms were exposed to the 410 nm LED lamp, microimages showed an increase of the propidium iodide-stained dead cells , which was further confirmed by cell counts using Imaris, where dead cells increased from 20% in the unilluminated control to more than 30% under 410 nm irradiation, but the difference was not statistically significant ( ).

Techniques: In Vitro, Irradiation, Staining, Flow Cytometry